Human Trabecular Meshwork Sphingolipid and Ceramide Profiles and Potential Latent Fungal Commensalism

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Comparative phospholipid profiles of control and glaucomatous human trabecular meshwork.

PURPOSE We compared phospholipid (phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, and phosphatidylinositol) profiles of control and glaucomatous trabecular meshwork (TM) derived from human donors. METHODS Control TM and most primary open angle glaucoma (POAG) TM were collected from cadaver donors. A select subset of POAG surgical TM samples also were collected for analyses....

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Sphingolipid and Ceramide Homeostasis: Potential Therapeutic Targets

Sphingolipids are ubiquitous in eukaryotic cells where they have been attributed a plethora of functions from the formation of structural domains to polarized cellular trafficking and signal transduction. Recent research has identified and characterised many of the key enzymes involved in sphingolipid metabolism and this has led to a heightened interest in the possibility of targeting these pro...

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Trabecular meshwork and outflow

Core Concepts • iOP elevation results from increased outflow resistance in the outflow pathways: the trabecular meshwork (TM) and the uveoscleral pathway. • steady-state iOP is found in normal eyes where iOP builds up in response to inflow of aqueous humour to the level sufficient to drive fluid across the resistance of the outflow pathways. • in most glaucomatous eyes, the resistance of the ou...

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Gene expression profiles of human trabecular meshwork cells induced by triamcinolone and dexamethasone.

PURPOSE Triamcinolone acetonide (TA) and dexamethasone (DEX) are corticosteroids commonly used for ocular inflammation, but both can cause ocular hypertension. In this study, the differential gene expression profile of human trabecular meshwork (TM) cells in response to treatment by TA in comparison with DEX was investigated. METHODS Total RNA was extracted from cultured human TM cells treate...

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PURPOSE To determine whether soluble CD44 (sCD44), a likely biomarker of primary open-angle glaucoma (POAG), is internalized in cultured human trabecular meshwork (TM) cells and trafficked to mitochondria. METHODS In vitro, 32-kD sCD44 was isolated from human sera, biotinylated, and dephosphorylated. TM cells were incubated for 1 hour at 4°C with biotinylated albumin (b-albumin), biotin-label...

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ژورنال

عنوان ژورنال: Investigative Opthalmology & Visual Science

سال: 2014

ISSN: 1552-5783

DOI: 10.1167/iovs.13-13570